{"id":23479,"date":"2021-11-04T12:09:07","date_gmt":"2021-11-04T12:09:07","guid":{"rendered":"https:\/\/evaggelatos.com\/?p=23479"},"modified":"2021-11-04T12:09:07","modified_gmt":"2021-11-04T12:09:07","slug":"%ce%bf%ce%b9-%cf%80%cf%81%cf%89%cf%84%ce%b5%ce%90%ce%bd%ce%b5%cf%82-%ce%b1%ce%ba%ce%af%ce%b4%ce%b1%cf%82-%cf%84%cf%8c%cf%83%ce%bf-%cf%84%ce%bf%cf%85-covid-19-%cf%8c%cf%83%ce%bf-%ce%ba%ce%b1%ce%b9","status":"publish","type":"post","link":"https:\/\/evaggelatos.com\/?p=23479","title":{"rendered":"\u039f\u03b9 \u03c0\u03c1\u03c9\u03c4\u03b5\u0390\u03bd\u03b5\u03c2 \u03b1\u03ba\u03af\u03b4\u03b1\u03c2 \u03c4\u03cc\u03c3\u03bf \u03c4\u03bf\u03c5 Covid-19 \u03cc\u03c3\u03bf \u03ba\u03b1\u03b9 \u03c4\u03c9\u03bd 4 \u03b5\u03bc\u03b2\u03bf\u03bb\u03af\u03c9\u03bd \u03b5\u03bc\u03c0\u03bf\u03b4\u03af\u03b6\u03bf\u03c5\u03bd \u03ad\u03c9\u03c2 \u03b2\u03bb\u03ac\u03c0\u03c4\u03bf\u03c5\u03bd \u03c4\u03bf\u03bd \u03bc\u03b7\u03c7\u03b1\u03bd\u03b9\u03c3\u03bc\u03cc \u03b5\u03c0\u03b9\u03b4\u03b9\u03cc\u03c1\u03b8\u03c9\u03c3\u03b7\u03c2 \u03c4\u03bf\u03c5 DNA"},"content":{"rendered":"
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Open Access<\/span>Article<\/span><\/div>\n

SARS\u2013CoV\u20132 Spike Impairs DNA Damage Repair and Inhibits V(D)J Recombination In Vitro<\/h1>\n
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by<\/p>\n

Hui Jiang<\/span><\/a><\/div>\n

1,2,*<\/sup><\/i> and <\/span><\/p>\n

Ya-Fang Mei<\/span><\/a><\/div>\n

2,*<\/sup><\/i><\/span><\/p>\n<\/div>\n

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1<\/sup><\/div>\n
Department of Molecular Biosciences, The Wenner\u2013Gren Institute, Stockholm University, SE-10691 Stockholm, Sweden<\/div>\n<\/div>\n
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2<\/sup><\/div>\n
Department of Clinical Microbiology, Virology, Ume\u00e5 University, SE-90185 Ume\u00e5, Sweden<\/div>\n<\/div>\n
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*<\/sup><\/div>\n
Authors to whom correspondence should be addressed.<\/div>\n<\/div>\n<\/div>\n<\/div>\n
Academic Editor: Oliver Schildgen<\/div>\n
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Viruses<\/em> 2021, 13<\/em>(10), 2056; https:\/\/doi.org\/10.3390\/v13102056<\/a><\/strong><\/span><\/div>\n
Received: 20 August 2021 \/ Revised: 8 September 2021 \/ Accepted: 8 October 2021 \/ Published: 13 October 2021<\/strong><\/span><\/div>\n
(This article belongs to the Special Issue SARS-CoV-2 Host Cell Interactions<\/a>)<\/div>\n
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Abstract (\u03a3\u03a5\u039d\u039f\u03a0\u03a4\u0399\u039a\u0391)<\/h2>\n<\/div>\n
Severe acute respiratory syndrome coronavirus 2 (SARS\u2013CoV\u20132) has led to the coronavirus disease 2019 (COVID\u201319) pandemic, severely affecting public health and the global economy. Adaptive immunity plays a crucial role in fighting against SARS\u2013CoV\u20132 infection and directly influences the clinical outcomes of patients. Clinical studies have indicated that patients with severe COVID\u201319 exhibit delayed and weak adaptive immune responses;<\/strong> however, the mechanism by which SARS\u2013CoV\u20132 impedes adaptive immunity remains unclear. Here, by using an in vitro cell line, we report that the SARS\u2013CoV\u20132 spike protein significantly inhibits DNA damage repair, which is required for effective V(D)J recombination in adaptive immunity.<\/strong> Mechanistically, we found that the spike protein localizes in the nucleus and inhibits DNA damage repair by impeding key DNA repair protein BRCA1 and 53BP1 recruitment to the damage site.<\/strong> Our findings reveal a potential molecular mechanism by which the spike protein might impede adaptive immunity and underscore the potential side effects of full-length spike-based vaccines.<\/div>\n
Keywords: <\/em> SARS\u2013CoV\u20132<\/a>; spike<\/a>; DNA damage repair<\/a>; V(D)J recombination<\/a>; vaccine<\/a><\/div>\n<\/div>\n<\/div>\n
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1. Introduction<\/h2>\n
Severe acute respiratory syndrome coronavirus 2 (SARS\u2013CoV\u20132) is responsible for the ongoing coronavirus disease 2019 (COVID\u201319) pandemic that has resulted in more than 2.3 million deaths<\/strong>. SARS\u2013CoV\u20132 is an enveloped single positive\u2013sense RNA virus that consists of structural and non\u2013structural proteins [1<\/a>]. After infection, these viral proteins hijack and dysregulate the host cellular machinery to replicate, assemble, and spread progeny viruses [2<\/a>]. Recent clinical studies have shown that SARS\u2013CoV\u20132 infection extraordinarily affects lymphocyte number and function [3<\/a>,4<\/a>,5<\/a>,6<\/a>]<\/strong>. Compared with mild and moderate survivors, patients with severe COVID\u201319 manifest a significantly lower number of total T cells, helper T cells, and suppressor T cells [3<\/a>,4<\/a>]. Additionally, COVID\u201319 delays IgG and IgM levels after symptom onset [5<\/a>,6<\/a>].<\/strong> Collectively, these clinical observations suggest that SARS\u2013CoV\u20132 affects the adaptive immune system. However, the mechanism by which SARS\u2013CoV\u20132 suppresses adaptive immunity remains unclear.<\/div>\n
As two critical host surveillance systems, the immune and DNA repair systems<\/strong> are the primary systems that higher organisms rely on for defense against diverse threats and tissue homeostasis. Emerging evidence indicates that these two systems are interdependent, especially during lymphocyte development and maturation [7<\/a>]. As one of the major double-strand DNA break (DSB) repair pathways, non-homologous end joining (NHEJ) repair plays a critical role in lymphocyte\u2013specific recombination\u2013activating gene endonuclease (RAG) \u2013mediated V(D)J recombination, which results in a highly diverse repertoire of antibodies in B cell and T cell receptors (TCRs) in T cells [8<\/a>]. For example, loss of function of key DNA repair proteins such as ATM, DNA\u2013PKcs, 53BP1, et al., leads to defects in the NHEJ repair which inhibit the production of functional B and T cells, leading to immunodeficiency<\/strong> [7<\/a>,9<\/a>,10<\/a>,11<\/a>]. In contrast, viral infection usually induces DNA damage via different mechanisms, such as inducing reactive oxygen species (ROS) production and host cell replication stress [12<\/a>,13<\/a>,14<\/a>]. If DNA damage cannot be properly repaired, it will contribute to the amplification of viral infection-induced pathology.<\/strong> Therefore, we aimed to investigate whether SARS\u2013CoV\u20132 proteins hijack the DNA damage repair system, thereby affecting adaptive immunity in vitro.<\/div>\n<\/section>\n
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2. Materials and Methods<\/h2>\n
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2.1. Antibodies and Reagents<\/h4>\n
DAPI (Cat #MBD0015), doxorubicin (Cat #D1515), H2<\/sub>O2<\/sub> (Cat #H1009), and \u03b2-tubulin antibodies (Cat #T4026) were purchased from Sigma-Aldrich. Antibodies against His tag (Cat #12698), H2A (Cat #12349), H2A.X (Cat #7631), \u03b3\u2013H2A.X (Cat #2577), Ku80 (Cat # 2753), and Rad51(Cat #8875) were purchased from Cell Signaling Technology (Danvers, MA, USA). 53BP1(Cat #NB100-304) and RNF168 (Cat #H00165918\u2013M01) antibodies were obtained from Novus Biologicals (Novus Biologicals, Littleton, CO, USA). Lamin B (Cat #sc\u2013374015), ATM (Cat #sc\u2013135663), DNA\u2013PK (Cat #sc\u20135282), and BRCA1(Cat #sc\u201328383) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). XRCC4 (Cat #PA5\u201382264) antibody was purchased from Thermo Fisher Scientific (Waltham, MA, USA).<\/div>\n<\/section>\n
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2.2. Plasmids<\/h4>\n
pHPRT\u2013DRGFP and pCBASceI were kindly gifted by Maria Jasin (Addgene plasmids #26476 and #26477) [15<\/a>]. pimEJ5GFP was a gift from Jeremy Stark (Addgene plasmid #44026) [16<\/a>]. The NSP1, NSP9, NSP13, NSP14, NSP16, spike, and nucleocapsid proteins were first synthesized with codon optimization and then cloned into a mammalian expression vector pUC57 with a C\u2013terminal 6xHis tag. A 12\u2013spacer RSS\u2013GFP inverted complementary sequence\u2013a 23\u2013spacer RSS was synthesized for the V(D)J reporter vector. Then, the sequence was cloned into the pBabe\u2013IRES\u2013mRFP vector to generate the pBabe\u201312RSS\u2013GFPi\u201323RSS\u2013IRES\u2013mRFP reporter vector. 12\u2013spacer RSS sequence: 5\u2032\u2013CACAGTGCTACAGACTGGAACAAAAACC\u20133\u2032. 23\u2013spacer RSS sequence: 5\u2032\u2013CACAGTGGTAGTACTCCACTGTCTGGCTGTACAAAAACC\u20133\u2032. RAG1 and RAG2 expression constructs were generously gifted by Martin Gellert (Addgene plasmid #13328 and #13329) [17<\/a>].<\/div>\n<\/section>\n
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2.3. Cells and Cell Culture<\/h4>\n
HEK293T and HEK293 cells obtained from the American Type Culture Collection (ATCC) were cultured under 5% CO2<\/sub> at 37 \u00b0C in Dulbecco\u2019s modified Eagle\u2019s medium (DMEM, high glucose, GlutaMAX) (Life Technologies, Carlsbad, CA, USA) containing 10% (v<\/span>\/v<\/span>) fetal calf serum (FCS, Gibco), 1% (v<\/span>\/v<\/span>) penicillin (100 IU\/mL), and streptomycin (100\u2009\u03bcg\/mL). HEK293T\u2013DR\u2013GFP and HEK293T\u2013EJ5\u2013GFP reporter cells were generated as previously described and cultured under 5% CO2<\/sub> at 37 \u00b0C in the above-mentioned culture medium.<\/div>\n<\/section>\n
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2.4. HR and NHEJ Reporter Assays<\/h4>\n
HR and NHEJ repair in HEK293T cells were measured as described previously using DR\u2013GFP and EJ5\u2013GFP stable cells. Briefly, 0.5 \u00d7 106<\/sup> HEK293T stable reporter cells were seeded in 6\u2013well plates and transfected with 2 \u03bcg I\u2013SceI expression plasmid (pCBASceI) together with SARS\u2013CoV\u20132 proteins expression plasmids. Forty\u2013eight hours post\u2013transfection and aspirin treatment, cells were harvested and analyzed by flow cytometry analysis for GFP expression. The means were obtained from three independent experiments.<\/div>\n<\/section>\n
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2.5. Cellular Fractionation and Immunoblotting<\/h4>\n
For the cellular fraction assay, the Subcellular Protein Fractionation Kit (Thermo Fisher) was used according to the manufacturer\u2019s instructions. Protein lysates were quantified using the BCA reagent (Thermo Fisher Scientific, Rockford, IL, USA). Proteins were resolved by sodium dodecyl sulfate\u2013polyacrylamide gel electrophoresis (SDS\u2013PAGE), transferred to nitrocellulose membranes (Amersham protran, 0.45 \u03bcm NC), and immunoblotted with specific primary antibodies followed by HRP\u2013conjugated secondary antibodies. Protein bands were detected using SuperSignal West Pico or Femto Chemiluminescence kit (Thermo Fisher Scientific).<\/div>\n<\/section>\n
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2.6. Comet Assay<\/h4>\n
Cells were treated with different DNA damage reagents and then harvested at the indicated time points for analysis. Cells (1 \u00d7 105<\/sup> cells\/mL in cold phosphate-buffered saline [PBS]) were resuspended in 1% low\u2013melting agarose at 40 \u00b0C at a ratio of 1:3 vol\/vol and pipetted onto a CometSlide. Slides were then immersed in prechilled lysis buffer (1.2 M NaCl, 100 mM EDTA, 0.1% sodium lauryl sarcosinate, 0.26 M NaOH pH > 13) for overnight (18\u201320 h) lysis at 4 \u00b0C in the dark. Slides were then carefully removed and submerged in rinse buffer (0.03 M NaOH and 2 mM EDTA, pH > 12) at room temperature (RT) for 20 min in the dark. This washing step was repeated twice. The slides were transferred to a horizontal electrophoresis chamber containing rinse buffer and separated for 25 min at a voltage of 0.6 V\/cm. Finally, the slides were washed with distilled water, stained with 10 \u03bcg\/mL propidium iodide, and analyzed by fluorescence microscopy. Twenty fields with approximately 100 cells in each sample were evaluated and quantified using the Fiji software to determine the tail length (tail moment).<\/div>\n<\/section>\n
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2.7. Immunofluorescence<\/h4>\n
Cells were seeded on glass coverslips in a 12\u2013well plate and transfected with the indicated plasmid for 24 h. Then, the cells were treated with or without DNA damage reagents according to the experimental setup. The cells were fixed in 4% paraformaldehyde (PFA) in PBS for 20 min at RT and then permeabilized in 0.5% Triton X\u2013100 for 10 min. Slides were blocked in 5% normal goat serum (NGS) and incubated with primary antibodies diluted in 1% NGS overnight at 4 \u00b0C. Samples were then incubated with the indicated secondary antibodies labeled with Alexa Fluor 488 or 555 (Invitrogen) diluted in 1% NGS at RT for 1 h. Thereafter, they were stained with DAPI for 15 min at RT. Coverslips were mounted using Dako Fluorescence Mounting Medium (Agilent) and imaged using a Nikon confocal microscope (Eclipse C1 Plus). All scoring was performed under blinded conditions.<\/div>\n<\/section>\n
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2.8. Analysis of V(D)J Recombination<\/h4>\n
Briefly, V(D)J reporter plasmid contains inverted-GFP and IRES driving continuously expressed RFP. Continuously expressed RFP is the internal transfection control. After Recombination activation gene1\/2 (RAG1\/2) co\u2013transfected into the cells, RAG1\/2 will cut the RSS and mediated induction of DSBs, if V(D)J recombination occurs, the inverted GFPs are ligated in positive order by NHEJ repair. Then the cell will express functional GFP. So, the GFP and RFP double positive cells are the readout of the V(D)J reporter assay [18<\/a>]. 293T cells at 70% confluency were transfected with the V(D)J GFP reporter alone (background) or in combination with RAG1 and RAG2 expression constructs, at a ratio of 1 \u00b5g V(D)J GFP reporter: 0.5 \u00b5g RAG1: 0.5 \u00b5g RAG2. The following day, the medium was changed, and after an additional 48 h, cells were harvested and analyzed by flow cytometry for GFP and RFP expression.<\/div>\n<\/section>\n
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2.9. Statistical Analysis<\/h4>\n
All experiments were repeated at least three times using independently collected or prepared samples. Data were analyzed by Student\u2019s t test or ANOVA followed by Tukey\u2019s multiple-comparison tests using GraphPad 8.<\/div>\n<\/section>\n<\/section>\n
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3. Results<\/h2>\n
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3.1. Effect of Nuclear\u2013Localized SARS\u2013CoV\u20132 Viral Proteins on DNA Damage Repair<\/h4>\n
DNA damage repair occurs mainly in the nucleus to ensure genome stability.<\/strong> Although SARS\u2013CoV\u20132 proteins are synthesized in the cytosol [1<\/a>], some viral proteins are also detectable in the nucleus, including Nsp1, Nsp5, Nsp9, Nsp13, Nsp14, and Nsp16 [19<\/a>]. We investigated whether these nuclear-localized SARS\u2013CoV\u20132 proteins affect the host cell DNA damage repair system. For this, we constructed these viral protein expression plasmids together with spike and nucleoprotein expression plasmids, which are generally considered cytosol\u2013localized proteins. We confirmed their expression and localization by immunoblotting and immunofluorescence (Figure 1<\/a>A and Figure S1A<\/a>). Our results were consistent with those from previous studies [19<\/a>]; Nsp1, Nsp5, Nsp9, Nsp13, Nsp14, and Nsp16 proteins are indeed localized in the nucleus, and nucleoproteins are mainly localized in the cytosol. Surprisingly, we found the abundance of the spike protein in the nucleus (Figure 1<\/a>A).<\/strong> NHEJ repair and homologous recombination (HR) repair are two major DNA repair pathways that not only continuously monitor and ensure genome integrity but are also vital for adaptive immune cell functions [9<\/a>]. To evaluate whether these viral proteins impede the DSB repair pathway, we examined the repair of a site-specific DSB induced by the I\u2013SceI endonuclease using the direct repeat\u2013green fluorescence protein (DR\u2013GFP) and the total-NHEJ-GFP (EJ5\u2013GFP) reporter systems for HR and NHEJ, respectively [15<\/a>,16<\/a>]. Overexpression of Nsp1, Nsp5, Nsp13, Nsp14, and spike proteins diminished the efficiencies of both HR and NHEJ repair (Figure 1<\/a>B\u2013E and Figure S2A,B<\/a>). Moreover, we also found that Nsp1, Nsp5, Nsp13, and Nsp14 overexpression dramatically suppressed proliferation compared with other studied proteins (Figure S3A,B<\/a>). Therefore, the inhibitory effect of Nsp1, Nsp5, Nsp13, and Nsp14 on DNA damage repair may be due to secondary effects, such as growth arrest and cell death.<\/strong> Interestingly, overexpressed spike protein did not affect cell morphology or proliferation but significantly suppressed both HR and NHEJ repair (Figure 1<\/a>B\u2013E, Figures S2A,B and S3A,B<\/a>).<\/div>\n
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Figure 1.<\/b> Effect of severe acute respiratory syndrome coronavirus 2 (SARS\u2013CoV\u20132) nuclear-localized proteins on DNA damage repair. (A<\/b>) Subcellular distribution of the SARS\u2013CoV\u20132 proteins. Immunofluorescence was performed at 24 h after transfection of the plasmid expressing the viral proteins into HEK293T cells. Scale bar: 10 \u00b5m. (B<\/b>) Schematic of the EJ5-GFP reporter used to monitor non-homologous end joining (NHEJ). (C<\/b>) Effect of empty vector (E.V) and SARS\u2013CoV\u20132 proteins on NHEJ DNA repair. The values represent the mean \u00b1 standard deviation (SD) from three independent experiments (see representative FACS plots in Figure S2A<\/a>). (D<\/b>) Schematic of the DR-GFP reporter used to monitor homologous recombination (HR). (E<\/b>) Effect of E.V and SARS\u2013CoV\u20132 proteins on HR DNA repair. The values represent the mean \u00b1 SD from three independent experiments (see representative FACS plots in Figure S2B<\/a>). The values represent the mean \u00b1 SD, n<\/span> = 3. Statistical significance was determined using one-way analysis of variance (ANOVA) in (C<\/b>,E<\/b>). ** p<\/span> < 0.01, *** p<\/span> < 0.001, **** p<\/span> < \u20090.0001.<\/div>\n<\/div>\n<\/section>\n
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3.2. SARS\u2013CoV\u20132 Spike Protein Inhibits DNA Damage Repair<\/h4>\n
Because spike proteins are critical for mediating viral entry into host cells and are the focus of most vaccine strategies [20<\/a>,21<\/a>], we further investigated the role of spike proteins in DNA damage repair and its associated V(D)J recombination. Spike proteins are usually thought to be synthesized on the rough endoplasmic reticulum (ER) [1<\/a>]. After posttranslational modifications such as glycosylation, spike proteins traffic via the cellular membrane apparatus together with other viral proteins to form the mature virion [1<\/a>]. Spike protein contains two major subunits, S1 and S2, as well as several functional domains or repeats [22<\/a>] (Figure 2<\/a>A). In the native state, spike proteins exist as inactive full\u2013length proteins. During viral infection, host cell proteases such as furin protease activate the S protein by cleaving it into S1 and S2 subunits, which is necessary for viral entry into the target cell [23<\/a>]. We further explored different subunits of the spike protein to elucidate the functional features required for DNA repair inhibition. Only the full\u2013length spike protein strongly inhibited both NHEJ and HR repair (Figure 2<\/a>B\u2013E and Figure S4A,B<\/a>). Next, we sought to determine whether the spike protein directly contributes to genomic instability by inhibiting DSB repair. We monitored the levels of DSBs using comet assays. Following different DNA damage treatments, such as \u03b3\u2013irradiation, doxorubicin treatment, and H2<\/sub>O2<\/sub> treatment, there is less repair in the presence of the spike protein (Figure 2<\/a>F,G). Together, these data demonstrate that the spike protein directly affects DNA repair in the nucleus.<\/div>\n
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Figure 2.<\/b> Severe acute respiratory syndrome coronavirus 2 (SARS\u2013CoV\u20132) spike protein inhibits DNA damage repair. (A<\/b>) Schematic of the primary structure of the SARS\u2013CoV\u20132 spike protein. The S1 subunit includes an N\u2013terminal domain (NTD, 14\u2013305 residues) and a receptor\u2013binding domain (RBD, 319\u2013541 residues). The S2 subunit consists of the fusion peptide (FP, 788\u2013806 residues), heptapeptide repeat sequence 1 (HR1, 912\u2013984 residues), HR2 (1163\u20131213 residues), TM domain (TM, 1213\u20131237 residues), and cytoplasm domain (CT,1237\u20131273 residues). (B<\/b>,C<\/b>) Effect of titrated expression of the spike protein on DNA repair in HEK\u2013293T cells. (D<\/b>,E<\/b>) Only full-length spike protein inhibits non-homologous end joining (NHEJ) and homologous recombination (HR) DNA repair. The values represent the mean \u00b1 SD from three independent experiments (see representative FACS plots in Figure S4A,B<\/a>). (F<\/b>) Full\u2013length spike (S\u2013FL) protein\u2013transfected HEK293T cells exhibited more DNA damage than empty vector-, S1\u2013, and S2\u2013transfected cells under different DNA damage conditions. For doxorubicin: 4 \u00b5g\/mL, 2 h. For \u03b3\u2013irradiation: 10 Gy, 30 min. For H2<\/sub>O2<\/sub>: 100 \u00b5M, 1 h. Scale bar: 50 \u00b5m. (G<\/b>) Corresponding quantification of the comet tail moments from 20 different fields with n<\/span> > 200 comets of three independent experiments. Statistical significance was assessed using a two-way analysis of variance (ANOVA). NS (Not Significant): * p<\/span> > 0.05, ** p<\/span> <\u20090.01, *** p<\/span> < 0.001, **** p<\/span> < 0.0001.<\/div>\n<\/div>\n<\/section>\n
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3.3. Spike Proteins Impede the Recruitment of DNA Damage Repair Checkpoint Proteins<\/h4>\n
To confirm the existence of spike protein in the nucleus, we performed subcellular fraction analysis and found that spike proteins are not only enriched in the cellular membrane fraction but are also abundant in the nuclear fraction, with detectable expression even in the chromatin\u2013bound fraction<\/strong> (Figure 3<\/a>A). We also observed that the spike has three different forms, the higher band is a highly glycosylated spike, the middle one is a full\u2013length spike, and the lower one is a cleaved spike subunit. Consistent with the comet assay, we also found the upregulation of the DNA damage marker, \u03b3\u2013H2A.X, in spike protein\u2013overexpressed cells under DNA damage conditions (Figure 3<\/a>B). A recent study suggested that spike proteins induce ER stress and ER\u2013associated protein degradation [24<\/a>]. To exclude the possibility that the spike protein inhibits DNA repair by promoting DNA repair protein degradation, we checked the expression of some essential DNA repair proteins in NHEJ and HR repair pathways and found that these DNA repair proteins were stable after spike protein overexpression (Figure 3<\/a>C). To determine how the spike protein inhibits both NHEJ and HR repair pathways, we analyzed the recruitment of BRCA1 and 53BP1, which are the key checkpoint proteins for HR and NHEJ repair, respectively. We found that the spike protein markedly inhibited both BRCA1 and 53BP1 foci formation (Figure 3<\/a>D\u2013G). Together, these data show that the SARS\u2013CoV\u20132 full\u2013length spike protein inhibits DNA damage repair by hindering DNA repair protein recruitment.<\/div>\n
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Figure 3.<\/b> Severe acute respiratory syndrome coronavirus 2 (SARS\u2013CoV\u20132) spike protein impedes the recruitment of DNA damage repair checkpoint proteins.<\/strong> (A<\/b>) Membrane fraction (MF), cytosolic fraction (CF), soluble nuclear fraction (SNF), and chromatin-bound fraction (CBF) from HEK293T cells transfected with SARS\u2013CoV\u20132 spike protein were immunoblotted for His-tag spike and indicated proteins. (B<\/b>) Left: Immunoblots of DNA damage marker \u03b3H2AX in empty vector (E.V)\u2013 and spike protein\u2013expressing HEK293T cells after 10 Gy \u03b3-irradiation. Right: corresponding quantification of immunoblots in left. The values represent the mean \u00b1 SD (n<\/span> = 3). Statistical significance was determined using Student\u2019s t<\/span>-test. **** p<\/span>\u2009< 0.0001. (C<\/b>) Immunoblots of DNA damage repair related proteins in spike protein\u2013expressing HEK293T cells. (D<\/b>) Representative images of 53BP1 foci formation in E.V\u2013 and spike protein-expressing HEK293 cells exposed to 10 Gy \u03b3\u2013irradiation. Scale bar: 10 \u00b5m. (E<\/b>) Quantitative analysis of 53BP1 foci per nucleus. The values represent the mean \u00b1 SEM, n<\/span> = 50. (F<\/b>) BRCA1 foci formation in empty vector- and spike protein-expressing HEK293 cells exposed to 10 Gy \u03b3\u2013irradiation. Scale bar: 10 \u00b5m. (G<\/b>). Quantitative analysis of BRCA1 foci per nucleus. The values represent the mean \u00b1 SEM, n<\/span> = 50. Statistical significance was determined using Student\u2019s t<\/span>-test. **** p<\/span> < 0.0001.<\/div>\n<\/div>\n<\/section>\n
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3.4. Spike Protein Impairs V(D)J Recombination In vitro<\/h4>\n
DNA damage repair, especially NHEJ repair, is essential for V(D)J recombination, which lies at the core of B and T cell immunity [9<\/a>]. To date, many approved SARS\u2013CoV\u20132 vaccines, such as mRNA vaccines and adenovirus\u2013COVID\u201319 vaccines, have been developed based on the full\u2013length spike protei<\/strong>n [25<\/a>]. Although it is debatable whether SARS\u2013CoV\u20132 directly infects lymphocyte precursors [26<\/a>,27<\/a>], some reports have shown that infected cells secrete exosomes that can deliver SARS\u2013CoV\u20132 RNA or protein to target cells [28<\/a>,29<\/a>]. We further tested whether the spike protein reduced NHEJ\u2013mediated V(D)J recombination. For this, we designed an in vitro V(D)J recombination reporter system according to a previous study [18<\/a>] (Figure S5<\/a>). Compared with the empty vector, spike protein overexpression inhibited RAG\u2013mediated V(D)J recombination in this in vitro reporter system (Figure 4<\/a>).<\/div>\n
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Figure 4.<\/b> Spike protein impairs V(D)J recombination in vitro. (A<\/b>) Schematic of the V(D)J reporter system. (B<\/b>) Representative plots of flow cytometry show that the SARS\u2013CoV\u20132 spike protein impedes V(D)J recombination in vitro. (C<\/b>) Quantitative analysis of relative V(D)J recombination. The values represent the mean \u00b1 SD, n<\/span> = 3. Statistical significance was determined using Student\u2019s t<\/span>-test. **** p<\/span> < 0.0001.<\/div>\n<\/div>\n<\/section>\n<\/section>\n
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4. Discussion<\/h2>\n
Our findings provide evidence of the spike protein hijacking the DNA damage repair machinery and adaptive immune machinery in vitro. We propose a potential mechanism by which spike proteins may impair adaptive immunity by inhibiting DNA damage repair. Although no evidence has been published that SARS\u2013CoV\u20132 can infect thymocytes or bone marrow lymphoid cells, our in vitro V(D)J reporter assay shows that the spike protein intensely impeded V(D)J recombination<\/strong><\/span>. Consistent with our results, clinical observations also show that the risk of severe illness or death with COVID\u201319 increases with age, especially older adults who are at the highest risk [22<\/a>]. This may be because SARS\u2013CoV\u20132 spike proteins can weaken the DNA repair system of older people and consequently impede V(D)J recombination and adaptive immunity.<\/strong> In contrast, our data provide valuable details on the involvement of spike protein subunits in DNA damage repair, indicating that full\u2013length spike\u2013based vaccines may inhibit the recombination of V(D)J in B cells, which is also consistent with a recent study that a full\u2013length spike\u2013based vaccine induced lower antibody titers compared to the RBD\u2013based vaccine<\/strong> [28<\/a>]. This suggests that the use of antigenic epitopes of the spike as a SARS\u2013CoV\u20132 vaccine might be safer and more efficacious than the full\u2013length spike. Taken together, we identified one of the potentially important mechanisms of SARS\u2013CoV\u20132 suppression of the host adaptive immune machinery. Furthermore, our findings also imply a potential side effect of the full\u2013length spike\u2013based vaccine. This work will improve the understanding of COVID\u201319 pathogenesis and provide new strategies for designing more efficient and safer vaccines.<\/div>\n<\/section>\n<\/div>\n
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Supplementary Materials<\/h2>\n
The following are available online at https:\/\/www.mdpi.com\/article\/10.3390\/v13102056\/s1<\/a>, Figure S1: Expression of nuclear\u2013localized SARS\u2013CoV\u20132 proteins in human cells, Figure S2: Effect of nuclear SARS\u2013CoV\u20132 proteins on NHEJ\u2013 and HR\u2013DNA repair pathway, Figure S3: Nsp1, Nsp5, Nsp13, Nsp14 but not spike inhibit cell proliferation, Figure S4: Effect of SARS\u2013CoV\u20132 spike mutants on NHEJ\u2013 and HR\u2013 DNA repair pathway, Figure S5: In vitro V(D)J recombination assay.<\/div>\n<\/section>\n<\/section>\n
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Author Contributions<\/h2>\n
H.J. conceived and designed the study. H.J. and Y.-F.M. supervised the study, performed experiments, and interpreted the data. Writing\u2014original draft preparation, H.J.; Writing\u2014review and editing, H.J. and Y.-F.M.; funding acquisition, Y.-F.M. All authors have read and agreed to the published version of the manuscript.<\/div>\n<\/section>\n
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Funding<\/h2>\n
This work was supported by Ume\u00e5 University, Medical Faculty\u2019s Planning grants for COVID\u201319 (research project number: 3453 16032 to Y.F.M.); the Lion\u2019s Cancer Research Foundation at Ume\u00e5 University (grants: LP 17\u20132153, AMP 19\u2013982, and LP 20\u20132256 to Y.F.M.), and the base unit\u2019s ALF funds for research at academic healthcare units and university healthcare units in the northern healthcare region (ALF\u2013Basenheten: 2019, 2020, 2021 to Y.F.M.).<\/div>\n<\/section>\n
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Institutional Review Board Statement<\/h2>\n
Not applicable, because of this study not involving humans or animals.<\/div>\n<\/section>\n
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Informed Consent Statement<\/h2>\n
Not applicable, because of this study not involving humans.<\/div>\n<\/section>\n
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Data Availability Statement<\/h2>\n
The data presented in this study are available in the main text and Supplementary Materials<\/a>.<\/div>\n<\/section>\n
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Conflicts of Interest<\/h2>\n
The authors have declared that no competing interests exist. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.<\/div>\n<\/section>\n
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    Open AccessArticle SARS\u2013CoV\u20132 Spike Impairs DNA Damage Repair and Inhibits V(D)J Recombination In Vitro by Hui Jiang 1,2,* and Ya-Fang Mei 2,* 1 Department of Molecular Biosciences, The Wenner\u2013Gren Institute, Stockholm University, SE-10691 Stockholm, Sweden 2 Department of Clinical Microbiology, Virology, Ume\u00e5 University, SE-90185 Ume\u00e5, Sweden * Authors to whom correspondence should be addressed. Academic … <\/p>\n

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